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Automated Orthogonal Deprotection of Glu(OAllyl) and Peptide Stapling via Lactamization

April 18, 2019
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Summary

 

Orthogonal deprotection of Glu(OAllyl) and peptide stapling via lactamization can be readily performed on the Liberty Blue automated microwave peptide synthesizer. Branched and lactam stapled variants of both the HIV-1 antibody epitope gp41659-671 and Afamelanotide were synthesized, with each of the four peptides synthesized between 80 – 87% purity and requiring 3 – 3.5 h of synthesis time, generating only 700 – 750 mL of total waste per synthesis.