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Automated In Situ Hybridization and Immunohistochemistry System
InsituPro
Automated In Situ Hybridization and Immunohistochemistry System
InsituPro
Overview
The InsituPro™ is a dedicated system for automated in situ hybridization (ISH) to prepare samples for detection of DNA or RNA(mRNA, miRNA) and also for immunohistochemistry (IHC) to detect proteins and other antigens. The system automates all of the tedious and repetitive washing and incubation steps for ISH and IHC processes.Features
- Works with whole mounts, vibratome sections, thin sections on slides, and cells on coverslips
- Used on wide range of organisms (mouse, zebrafish, chicken, Xenopus, Drosophila, Arabidopsis, and many more)
- Fully customizable programs
- 16 buffer positions (2 cooled for preserving delicate antibodies)
- Allows partial recovery of expensive antibodies and probes
Flexibility
Use Whole Mounts and Slides
Baskets |
Slides |
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60 x (75mm x 25mm x 1.0mm) |
Whole mount preparations and vibratome sections are processed in separate incubation baskets in keyhole shaped cavities in the specimen tray. The specimen tray allows for heating and cooling between 6°C – 75°C and provides very gentle liquid exchange for even the most delicate samples of the specimens.
Slides with thin sections are mounted on a counter slide forming an independent incubation chamber. Six of these chambers are combined to a slide block and ten of these blocks are mounted vertically into the heated incubation tub. The incubation tub allows for control of temperature and high humidity for optimal performance. Liquid exchange is driven by gravity flow and capillary forces ensure the sections are submerged in buffer.
Established System
Widely Used
The InsituPro has a strong record for In situ Hybridization and Immunohistochemistry.- Used at > 300 research groups worldwide
- Cited in > 550 publications
- Applicable to zebrafish, mouse, Ababidopsis, Drosophila, Xenopus, chicken, and more
Open System
Low Running Costs
The InsituPro does not require the use of special kits and allows for standard lab buffers to be used. Additionally, the system allows for partial recovery of expensive probes and antibodies that can then be reused for subsequent samples. These features make operating the InsituPro very cost efficient.
