Automated Microwave-Enhanced Synthesis of Glycopeptides with O-Linked Glycans

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Glycopeptides containing O-linked glycans can be synthesized quickly with good purity using microwave-enhanced SPPS. Preparation of a peptide substrate of WbwA (a sialic acid glycosyltransferase), was completed in 3 h 10 min with 68% purity on the Liberty Blue automated microwave peptide synthesizer. Synthesis of an analog of APF (antiproliferative factor) conjugated to the TUS nuclear localization sequence was achieved in 1 h 8 min with 73% purity using the Liberty PRIME automated microwave peptide synthesizer.