Automated Synthesis of Cyclic Disulfide-Bridged Peptides

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Cyclic disulfide-bridged peptides can be prepared rapidly with good purity using microwave-enhanced SPPS on the Liberty Blue automated microwave peptide synthesizer. Synthesis of the peptide hormone oxytocin was achieved in under 3 h with 69% purity. Preparation of a peptide agonist of BMP receptor activin-like kinase 3 (Alk3), THR-123, was completed in 3 h with 77% purity. CHEC-7, a neuroprotective peptide inhibitor of amyloidogenesis, was prepared in under 3 h with 80% purity. Finally, a peptide venom from cone snails containing two disulfide bridges (conotoxin-SI) was synthesized in under 4 h with 67% purity.