Automated Synthesis of Hydrocarbon-Stapled Peptides Via Microwave Assisted Ring-Closing Metathesis

Download File


Hydrocarbon-stapled peptides can be synthesized rapidly with excellent purity using microwave enhanced SPPS on the Liberty Blue™ automated microwave peptide synthesizer. Synthesis of a pro-apoptotic BID stapled peptide derivative, BID SAHB (stabilized alpha-helix of BCL-2 domain), was achieved in under 4 h with 80% purity. Preparation of a pro-apoptotic BIM stapled peptide, BIM SAHB, was completed in under 4 h with 80% purity.