High Purity Peptide Synthesis Capable of Achieving Efficient Milligram to Kilogram Scale with cGMP Methodology




Overview

Available On Demand
Airdate: Wednesday, May 12, 2021
Duration: 1 Hour

Summary

The use of high efficiency solid phase peptide synthesis (HE-SPPS) allows synthesis of peptides at a previously unprecedented level of high speed, high purity, and low production of chemical waste. These developments allow the production of a difficult 10 mer peptide (0.1mmol scale) in < 30 minutes with < 100mL total waste. This technology can be readily extended to produce a 50 mer peptide in 3.5 hours. It has also permitted scale up into the kilogram range with ease.

In this presentation, the most recent advances in microwave assisted SPPS are reported. Topics include: (1) review of current methodology, (2) introduction of new methodology for GMP high-throughput synthesis, and (3) scale up from milligram to kilogram quantities of peptide. The robust nature of these methodologies is demonstrated with the high-purity syntheses of a number of pharmaceutically relevant peptides as well post-synthetically sequences.

What You’ll Learn

  • Learn about how modern instrumentation revolutionizes peptide synthesis
  • See how large scale cGMP peptide synthesis is achievable with microwave technology
  • Document impurity profiles in days instead of weeks
  • Discover how to offer next day custom and catalogue SPPS services

Who Should Attend

  • Laboratory managers
  • Peptide chemists
  • Research scientists
  • Professors
  • Graduate students
  • Medical Researchers

Speakers

Giorgio Marini
Life Science Technical Specialist
CEM Corporation