Microwave Peptide Synthesizer
Liberty PRIME 2.0

Unparalleled speed and efficiency.

 

Overview


The Liberty PRIME™ 2.0 is the absolute best peptide synthesizer available in the world. The system features a unique one-pot deprotection and coupling process allowing for a remarkable 2 minute cycle time with only 8.5mL waste per cycle (at 0.1mmol). A key improvement in the 2.0 version of the Liberty PRIME is the ability to make cleaner and longer peptides with a new level of robustness than previously possible.

The Liberty PRIME 2.0 provides the ultimate flexibility for long, difficult, or complex peptides. Additionally, the system provides ultra-fast sequential synthesis workflow that can dramatically outperform traditional parallel peptide synthesizers. The Liberty PRIME 2.0 can be utilized in cGMP peptide production.



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Unique Chemistry

  • Microwave deprotection and coupling (patented)
  • CarboMAX™ coupling chemistry (patented)
  • One-pot coupling and deprotection chemistry (patented)
  • Nitrogen Headspace Flushing (patent pending)

Features

  • Unprecedented 2.5 min cycle time (at 0.1mmol)
  • Unrivaled 8.5mL waste per cycle (at 0.1mmol)
  • Precise calibration-free delivery of all reagents
  • True Internal fiber-optic temperature control
  • 0.005 - 5 mmol scale range w/ digital N2 control
  • 30 amino acid positions
  • Integrated RV Camera
  • High-throughput upgrade options (HT4, HT12, HT24)
  • Ability to use in cGMP peptide production

Unmatched Quality

A new level of peptide purity.

The Liberty PRIME 2.0 provides the ability to make even higher purity peptides than previously possible with the original Liberty PRIME system. This is based on advances in the system fluidics, reaction vessel environment, and chemistry methodology.

  • Optimized microwave chemistry methodology with ProTide resins
  • Cleanest reaction environment with updated headspace flushing technology
  • More direct optimized flow paths
  • Digitally variable N2 control for optimized bubbling, purging, and draining
liberty-prime-2-barstar
Barstar
Length: 89 amino acids
Synthesis Time: 11 hrs. 26 min.
Waste Generated: 1660 mL
Scale: 0.05 mmol


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liberty-prime-2-barstar
HIV Protease
Length: 99 amino acids
Synthesis Time: 12 hrs. 48 min.
Waste Generated: 1829 mL
Scale: 0.05 mmol


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liberty-prime-2-barstar
MDM2
Length: 127 amino acids
Synthesis Time: 16 hrs. 26 min.
Waste Generated: 2316 mL
Scale: 0.05 mmol


View Chromatogram
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Unrivaled Speed

Unrivaled Speed

Synthesize 20mer’s every 45 minutes.

Based on a revolutionary one-pot deprotection and coupling process, along with the patented CarboMAX coupling chemistry, an unprecedented 2 minute cycle time is achieved.


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Solvent

Solvent

Reduce SPPS chemical waste to unbelievable levels.

By using a patented process that reuses the coupling solution for the subsequent deprotection step, along with a system design that requires no internal washing the Liberty PRIME 2.0 reduces waste to unimaginable levels.

Up to 50% Less Waste

The Liberty PRIME 2.0 produces significantly less waste than the highly efficient Liberty Blue 2.0!



Advanced Reagent Delivery System

Advanced Reagent Delivery System

No calibration is needed.

All reagents are delivered either by pressure-based Flex-Add delivery system or precalibrated pumping system.

This means you can always be confident that the right amount of each reagent is delivered every time at volumes as low as 0.25mL. The Liberty PRIME 2.0 provides calibration-free reagent delivery that is ideal for GMP processes.


CarboMAX

CarboMAX

The best coupling method for peptide synthesis.

  • The fastest and most complete coupling
  • Stabilizes acid sensitive linkages which are problematic for standard carbodiimide chemistry (phospho, glyco, hyper-acid linkages)
  • Unmatched low-level of epimerization for all 20 amino acids due to faster activation (faster o-acylisourea formation)

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High Throughput (HT) Loaders

High Throughput (HT) Loaders

High-throughput peptide synthesis made easy.

  • Automated synthesis of 4, 12, or 24 peptides (individual 20mer’s can be completed every hour)
  • The Liberty PRIME 2.0 paired with either the HT4, HT12, or HT24 outperforms conventional parallel systems

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Software

Flexible, Powerful Software

The newly updated software on the Liberty PRIME 2.0 is both flexible and easy to use, making sure nothing stands between you and your synthesis. Customize methods with unique monomers, orthogonal deprotection, branching, cyclizing, and more. Easily check on reactions on the run screen, where the run can be edited, paused, and resumed at any time.



Method Editor

Easy and Flexible Sequence Programming

The method editor on the Liberty Blue allows full customization of reaction parameters.

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Alternate Chemistry and Post-Synthetic Modification

Program Post-Synthesis Modification

Versatile options enabling orthogonal deprotection for modifications like branching and cyclization.

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Calculator for Reagents

Full Suite of Calculator Tools

Convenient calculators provide the reagents and amounts for a run.

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Edit on the Run

Pause, Edit, Monitor at any Step

Easily pause, edit, and monitor reactions from the run screen.

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Calculator for Reagents

Audit Trail

Equipped with audit trail, the Liberty Prime 2.0 software time/date stamps all changes, runs, maintenance operations, etc.

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Edit on the Run

21 CFR Part 11 Compliant

The Liberty Prime 2.0 software is 21 CFR part 11 compliant, ready for GMP laboratory environments.

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Applications

Synthesize standard and complex peptides.

The Liberty 2.0 series, like preceding models, utilizes CEM’s High-Efficiency Solid Phase Peptide Synthesis (HE-SPPS) and CarboMAX coupling methodologies.

Branched Peptides
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Cyclized Peptides
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Disulfide Bonding
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Glycopeptides (N/O-linked)
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High Throughput Synthesis
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N-Methyl Peptides
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Peptoids
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Phosphopeptides
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Accessories and Related Products



HT 4

HT4

The HT4 automates peptide synthesis with the ability to queue up to 4 peptides. New reactions can be programmed at any time, even while another sample is running.

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HT 4

HT12

The HT12 option for the Liberty Blue and Liberty Prime provides automation for up to 12 peptides. New peptides can be added and programmed at any time, even while another sample is running.

Learn More
HT 4

HT24

The HT24 option provides automated synthesis of up to 24 peptides. The HT24 requires the HT12 and allows for 12 more peptides to be added to a queue (24 total). The HT24 is recommended only for the Liberty PRIME 2.0 system.

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Razor

Razor

The Razor from CEM provides high temperature, rapid cleavage on up to 12 samples simultaneously. Finish cleavage in just 30 minutes with the Razor.

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Prodigy

Prodigy

The Prodigy Preperative HPLC is a purification system optimized for use with biomolecules like peptides and proteins. The fully integrated temperature control for the column and mobile phase is a powerful tool, especially for difficult or hydrophobic samples

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