Microwave Assisted SPPS of Unsymmetrically Branched Peptides

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Microwave-enhanced SPPS allows unsymmetrically branched peptides to be synthesized rapidly and in high purity. Synthesis of a lactoferricin-lactoferrampin antimicrobial peptide (LF Chimera) was achieved in under 5 h with 77% purity. Synthesis of a histone H2B-1A peptide fragment (residues 118-126) conjugated to a ubiquitin peptide fragment (residues 47-76) was completed in under 5 h with 75% purity. Synthesis of tetrabranched analog of an antifreeze peptide was carried out in under 5 h with 71% purity.  All peptides were synthesized on the Liberty Blue automated microwave peptide synthesizer.