Validation of Universal Fat Analysis Method using Certified Reference Materials

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Abstract

Certified reference materials (CRMs) for food analysis are created and analyzed by trusted laboratories using known, validated test methods and offer insights into the inherent variation of a given analysis. They are considered a gold standard for comparative analysis and have safeguards in place to avoid method errors commonly encountered in a production testing environment. In this application note, we highlight the results of a blind comparison study, where a wide variety of certified reference materials were gathered, then tested in the ORACLE™. This study found that the ORACLE is capable of matching certified reference material values without any method development, calibration, or prior knowledge of the samples.

Introduction

The ORACLE universal fat analyzer is a low-resolution, timedomain NMR instrument (TD-NMR). However, unlike other TDNMR instruments that rely on free induction decay (FID) or spin echo pulse sequences, the ORACLE uses a proprietary train of pulses, which not only provides sufficient resolution to separate the fat signal from the signal of other components, but it also normalizes fat response, so that any fat molecule, regardless of source, results in a uniform signal. Because of these two properties, the ORACLE is able to accurately measure fat content in any food sample with no calibration or method development. As part of the ORACLE validation process, nearly 40 different certified reference materials (CRMs) were gathered and analyzed on the ORACLE.

CRMs were chosen for a variety of sample types and purchased from a variety of suppliers to show broad applicability. The study was conducted in a blind fashion where the operator was not given any information on the sample being analyzed.