Using Elevated Temperatures to Enable the Purification of Hydrophobic and Difficult Peptides




Overview

Available On Demand
Air Date: Tuesday, April 4th 2023
Time: 11:30 AM - 12:30 PM EDT

Summary

In this webinar, the successful purification of hydrophobic and difficult peptides is demonstrated using elevated temperature preparative HPLC. Learn how the use of an integrated heating system enables both the direct translation of elevated temperature methods to the preparative scale, as well as improved purity and recovery. Additionally, the benefits of Waters BEH reversed-phase columns for elevated temperature applications will be described.

Speakers

Colin L. Simpson
Principal Scientist
CEM Corporation

Colin Simpson is a Principal Scientist at CEM Corporation, and has over 12 years of experience developing and introducing improved technologies that serve the analytical testing and life science markets. In his current role, he is responsible for leading the development of improved approaches for peptide purification with a focus on both workflow solutions and instrumentation. He earned a B.A. in chemistry from UNC-Chapel Hill and a M.S. in bioanalytical chemistry from UNC-Charlotte.
 
Kathy Lawrence, M.S., MBA
Sr. Product Marketing Manager Consumables and Lab Automation
Waters Corporation

Kathy Lawrence is the Product Marketing Manager for Waters’ prep column technologies globally. Early on, she worked as both a discovery R&D chemist for ag chem and pharma where she gained valuable experience synthesizing small molecule insecticides and potential medicinal therapies. During this time, she learned to really leverage the power of various liquid chromatographic techniques to purify target bioactives, evaluate physical properties of the same and develop new skills isolating natural products. Later, in a contract research environment, she successfully managed an analytical-services laboratory supporting discovery synthesis. Kathy worked consultatively with labs across numerous industries during her tenure as Waters North American Purification Specialist where she supported the success of Water’s Prep solutions. Since then, Kathy has held increasingly advanced leadership roles in marketing and business development in support of the commercialization of purification solutions including instruments, consumables, lab services, and biotechnologies. Kathy holds a graduate degree in Chemistry, an MBA, and is she is an author of a handful of journal publications in addition to being a patented product inventor.

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