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Automated Enzymatic Digestion System
DigestPro
Automated Enzymatic Digestion System
DigestPro
Overview
The DigestPro™ is a dedicated protein digestion and sample preparation system for MS based proteomics. It completely automates all essential steps of protein digestion protocols and easily works with both in-solution and in-gel based methods.In addition sample clean-up and MALDI spotting can be automated. It achieves high throughput with up to 96 samples at a time. The whole procedure is performed under controlled and reproducible conditions that is essential for protein analysis by mass spectrometry.
Features
- Automated in-gel or in-solution digestion
- Ready-to-run optimized programs with full customizability
- Freely selectable temperature for each step (heating and cooling)
- Use of standard lab buffers and solutions (low running costs)
- Desalting and concentration with ZipTips (or AttractSPE Tips and similar reversed phase tips)
- MALDI target spotting
- Modular work area setup for individual instrument configuration
Simplified Proteomics
Easily Prepare Samples For Protein Analysis By Mass Spectrometry
Solution Digestion
- 1Reduction of disufide bonds
- 2Alkylation of cysteine residues/div>
- 3
Enzymatic Digestion (up to 70°C)- 4
Clean Up and Enrichment with Zip Tips or StageTipsIn-Gel Digestion
- 1Reduction of disufide bonds
- 2Alkylation of cysteine residues
- 3Enzymatic Digestion (up to 70°C)
- 4Washing of gel slices and elution of peptides. The needle adds liquids from a distance, never touching the sample and thus avoiding any risk of cross contamination.
- 5Clean Up and Enrichment with ZipTips or StageTips
- 3
In-Gel Digestions
Robust And Simple
Draining
- 1Air Purging
- 2Sealing Membrane
- 3Gel Slices
Addition of New Buffer
- 4Liquid Delivery
Sample Clean-Up
Automated Tip Assisted Desalting And Enrichment
The DigestPro allows for automated use of ZipTips, AttractSPE C18 Tips or similar reversed phase tips for easy clean-up and enrichment of samples (ZipTips are registered by EMD Millipore, AttractSPE Tips are registered by Affinisep). Tips are stored in a rack and are picked up and deposited onto the needle for repeated access. The tips enable peptide binding onto a C-18 substrate that allows for washing on the tip. The peptide solution can then be eluted with a small, concentrated volume of solution. Optionally, samples can directly be spotted onto MALDI targets with the tips. As another alternative to ZipTips or AttractSPE Tips, also selfmade StageTips can be used with the DigestPro.
View Applications Data
Enhanced Digestion
Rapid Digestion At Elevated Temperature
The DigestPro allows for rapid enzymatic digestion using programmable temperatures from 8 – 65°C. More rapid and complete digestions can be achieved with optimized elevated temperature protocols. Additionally, elevated temperature protocols can be used to simplify reduction of disulfide bonds.
Customizable Work Area
Design Your Work Area To Meet Your Needs
The DigestPro allows for full customizability of the work area. The customer can choose different modules required for his individual applications.
Modules are available for:
- In-gel digestion
- Solution digestion
- Sample clean-up on tips
- MALDI spotting
Furthermore, the flexibility includes the use of different vial formats for digestion and collection, programmable temperatures from 8 – 70°C, the usage of ZipTips, AttractSPE Tips or StageTips, and many available reagent positions.