The Prodigy™ is a unique preparative scale HPLC with a fully integrated heating system to access elevated temperatures for improved performance with peptide purification. This advanced heating system provides a uniform temperature for both the column and mobile phases and is compatible with columns up to 50 mm in diameter. Chromatographic methods from the heated analytical scale can now be performed directly at the preparative scale without the need for scouting runs through the use of temperature matching and CEM's proprietary focused gradient calculator.
The peptide focused development of the Prodigy makes it the ideal choice for simple or difficult separations - even for very hydrophobic peptides. The fully integrated heating system provides high level efficiency, without unnecessary complication.
- Fully integrated heating system with pre-optimized methods
- Built-in focused gradient calculator to convert from heated analytical to heated preparative scale
- Purify up to 1.5 grams per run of crude peptide
- UV directed fraction collection
- Intuitive software with one touch startup, collection, and cleaning
Powerful Peptide Purification
Optimized to be the Best in Peptide Purification
The Prodigy Preparative HPLC system is engineered for the best resolution of biomolecules and synthetic peptides. Key to system performance is its unique elevated temperature chromatography that provides improved separation capabilities beyond traditional preparative HPLC. This technology is supported by CEM’s proprietary focused gradient calculator that delivers optimized method transfer from analytical to preparative scale without the need for re-optimization or scouting runs. With the Prodigy, every chemist can improve resolution and recovery while saving time and solvent.
Resolve More Impurities
Separate Even Single Insertions
Analytical HPLC for peptides is performed at elevated temperature for the best resolution. Why not get the same great results for preparative separations? The fully integrated column heating system of the Prodigy HPLC translates this technology to the preparative scale with fully optimized methods designed by CEM peptide scientists. With the Prodigy, every chemist has the ability to better resolve closely eluting impurities.
Intrepid Column C18, 4.6 x 150 mm, 3 μm, 100A.
Method: 5-70%B (0.1% TFA in ACN) over 15 minutes, 1 mL/min, 220 nm.
Heated Preparative Chromatography
Excellent Separation of Hydrophobic Peptides
Hydrophobic peptides pose a unique challenge in reversed phase purification
due to limited solubility in the mobile phase. This challenge leads to poor
interaction with the stationary phase and poor resolution and peak shape in
chromatograms. The fully integrated heating system of the Prodigy increases
solubility of hydrophobic peptides and improves both resolution and peak shape
for the best chromatographic results.
Effortless Method Transfer
Make yours the most efficient peptide production workflow by eliminating optimization and scouting runs. CEM’s proprietary focused gradient calculator simplifies the method transfer process from analytical to preparative scale, removing time intensive and wasteful optimization steps common to non-heated chromatography workflows. This industry exclusive feature is included with Prodigy Preparative HPLC software along with the easiest to use programming interface.
User-Friendly Software, Scientist Centered Design
The new Prodigy icon driven software makes running a separation fast, easy, and efficient. The built in focused gradient calculator converts from heated analytical to heated preparative scale without the need for scouting runs. The powerful software on the Prodigy makes anyone a chromatographer.
The System Overview Window provides a quick summary of the instrument status.
Enter the parameters from your analytical run into the Focused Gradient Calculator.
The Focused Gradient calculator will automatically convert your analytical method to preparative parameters.
Select a method and update your parameters then touch the Start button to begin.
Customize your Prodigy
High Flow Configuration
For those with higher throughput needs, the Prodigy has a high flow configuration available. This system allows flow rates up to 250 mL/min, access to larger 50 mm diameter columns, and up to 1.5 grams of material for purification.
CEM Intrepid™ Columns
CEM offers high quality Intrepid brand columns for
analytical and preparative scale HPLC. Intrepid
columns are manufactured under tight controls to
provide narrow particle size distributions and minimal
back pressure. Ensure the best results with the Intrepid’s 5 um particle size options for preparative HPLC columns.
A Comprehensive Synthesis Solution
In addition to peptide purification solutions,
CEM is the industry leader in peptide synthesis
instrumentation. The Liberty and MultiPep product lines can cover any application, scale,