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Peptide Workflow
![CEM at Peptide Workflow](https://cem.com/media/contentmanager/content/media-library/headers/peptide-workflow-header.jpg)
1. Peptide Synthesis
Conventional synthesis workflows using traditional SPPS methodologies can be time consuming and require large amounts of solvents.CEM offers an ultra-fast sequential synthesis workflow (cycle times as short as 3 minutes) that is also ultra-efficient, massively reducing solvent usage compared to traditional synthesizers. This technology is known as Ultra-Efficient SPPS (UE-SPPS) and allows for the elimination of repetitive washing steps during each cycle. This advancement is based on chemical methodology improvements as well as a new headspace gas flushing technology for removal of volatile deprotection base at high temperature.
UE-SPPS is available on the Liberty Blue 2.0 and Liberty Prime 2.0.
Traditional SPPS Cycle (Extensive Wash Related Waste)
![Traditional SPPS Cycle Extensive wash related waste](https://cem.com/media/contentmanager/content/media-library/peptide-workflow-traditional.png)
UE-SPPS Cycle (No Wash Related Waste)
![UE-SPPS Cycle No wash related waste](https://cem.com/media/contentmanager/content/media-library/peptide-workflow-ue-spps.png)
UE-SPPS Examples
![Acyl Carrier Protein (ACP) figure UE-SPPS Example of Acyl Carrier
Protein (ACP)](https://cem.com/media/contentmanager/content/media-library/peptide-workflow-acyl-carrier-protein.png)
Acyl Carrier Protein (ACP)
Length: 10 amino acidsSynthesis Time: 30 min.
Waste Generated: 47 mL
![UE-SPPS Example of Proinsulin
figure Proinsulin figure](https://cem.com/media/contentmanager/content/media-library/peptide-workflow-proinsulin.jpg)
Proinsulin
Length: 86 amino acidsSynthesis Time: 10 hrs. 40 min.
Waste Generated: 677 mL
2. Cleavage
The cleavage step can be an underappreciated part of the peptide workflow, due to limited perceived opportunities for both performance and workflow improvements.CEM offers an elevated temperature cleavage system (Razor) that can simultaneously cleave 12 peptides in 30 minutes, eliminating the need for time consuming room temperature cleavages.
Using the Razor, you can cleave as fast as you can purify. at high temperature.
![Razor cleavage figure Cleave peptides with a CEM
Razor in 30 minutes](https://cem.com/media/contentmanager/content/media-library/peptide-razor-cleavage_1.png)
Peptide: HIV-TAT (47-57); Sequence: Fmoc-YGRKKRRQRRR
Conditions: TFA/TIS/H20/DODT (92.5/2.5/2.5/2.5)
3. Purification
The purification step can often be the bottleneck in peptide workflows due to slow and inefficient room temperature approaches that frequently require additional polishing steps.CEM offers a preparative HPLC instrument (Prodigy) featuring a unique integrated heating system, which allows for high efficiency direct scale up from elevated temperature analytical runs (up to 80 °C).
The use of elevated temperatures enables maximized productivity through shorter methods (due to higher flow rates) and improved fraction purity and recovery (due to improved peak shape).
![Prodigy purification figure Prodigy improved peak shape](https://cem.com/media/contentmanager/content/media-library/peptide-workflow-purification_1.png)