Automated Pressurized Extraction and In-Cell Cleanup for Mycotoxins, Veterinary Drugs, and Pesticides in Honey

Abstract

Honey is imported from all around the globe, and requires monitoring for a variety of unwanted mycotoxins, pesticides, veterinary drug residues. These residues cover a wide range of different chemistries and are typically part of a complex matrix, making extraction and analysis challenging. Herein, we provide an automated multi-residue extraction method for mycotoxins, veterinary drugs, and pesticides in honey using the EDGE®automated solvent exaction system. In this method, extraction, in-cell cleanup, and filtration are all accomplished in under 10 minutes. Following extraction, spiked samples were centrifuged and analyzed by LC-MS/MS through direct injection. Overall, excellent recoveries and reproducibility were achieved for the contaminants analyzed. The EDGE system offers a fast, reliable, and scalable method and workflow suitable for multi-class residue analysis in honey.



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