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Automated Deprotection of Orthogonal and Non-Standard Lysine Protecting Groups

July 20, 2018
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Summary

The Liberty Blue can perform automated deprotection of orthogonal and non-standard side-chain protecting groups, enabling side-chain branching, bioconjugation and cyclization. Microwave-enhanced SPPS on the Liberty Blue produced branched variants of the peptide gp41659–671, by Lys(Mmt)-, Lys(Alloc)-, and Lys(ivDde)-deprotection; subsequent coupling produced the variants in 79%, 82%, and 93% purity respectively. The three gp41659–671 variants were synthesized in a combined time of 9 h 15 min and required less than 1200 mL main wash (DMF).