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Complete Amino Acid Analysis of Foods and Feeds

August 24, 2016
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Amino acid analysis is used for the quantification of one group
of essential nutrients in foods and feeds. To provide a robust,
reproducible and accurate method for the quantification of all
the amino acids, both sample handling and chromatographic
separation must be taken into consideration. In sample
handling, the samples must be hydrolyzed so that the
proportions of amino acids can be measured. Multiple
hydrolysis approaches must be used because all amino acids
are not equally stable. Each sample must be subjected to 3
different hydrolysis protocols namely acid hydrolysis used to
determine the total protein composition; acid hydrolysis
following performic acid oxidation required to measure sulfur
containing amino acids and alkaline hydrolysis used to assess
tryptophan recovery. Sample hydrolysis is a time consuming
process requiring special equipment. Microwave hydrolysis
implementation for all three protocols results in improved
control of hydrolysis conditions with better accuracy,
reproducibility, speed and robustness. In this work we show
the implementation of all three hydrolysis protocols for raw
feeds, such as, soy bean meal, as well as complete mixed
feeds. Chromatographic separation is used to identify and
quantify the released amino acids. Reversed-phase UPLC® of
amino acids derivatized with 6-aminoquinolyl-Nhydroxysuccinimidyl
(AQC) provides high resolution and