Towards an Optimized Approach for Peptide Purification at Elevated Temperature


Available On Demand
Airdate: Wednesday, September 14, 2022
Duration: 1 Hour

Recorded live at the American Peptide Society 2022 meeting in Whistler.


Advances in synthesis methodologies and an increased interest in the therapeutic potential of peptides have put a renewed emphasis on improvements in purification speed and performance. Despite this, the use of elevated temperatures is often overlooked in HPLC purification workflows, even as potential performance benefits are well understood. We present here a review of elevated temperature HPLC concepts as well as proposed approaches for optimization of purification workflows at elevated temperature.

What You’ll Learn

  • An Overview of HPLC Fundamentals
  • Balancing Throughput, Purity, and Yield in Purification
  • Conditions for Operating at Elevated Temperature
  • Case Studies for Peptide Purification

Who Should Attend

  • Peptide Scientists
  • Purification Scientists
  • Peptide and Protein Researchers


Colin L. Simpson
Senior Research Scientist
CEM Corporation

Equipment Used